[rat-forum] PTH Levels in SD Rat

Raff, Hershel hraff at mcw.edu
Tue Feb 7 10:21:40 CST 2012


  I agree - the "Construction" paper I attached in my previous email showed exactly that.  I have never seen PTH values that high except in raging primary hyperparathyroidism and really bad renal failure (secondary hyperparathyroidism).  My gut feeling is there is something wrong with the assay.  HR

________________________________
From: rat-forum-bounces at mcw.edu [rat-forum-bounces at mcw.edu] On Behalf Of Michael Tordoff [tordoff at monell.org]
Sent: Tuesday, February 07, 2012 10:07 AM
To: rat-forum
Subject: Re: [rat-forum] PTH Levels in SD Rat

Hi Rajesh:

In my lab, PTH levels of calcium-replete Sprague Dawley rats are around 30 pg/ml and moderately calcium-deprived rats are 100 – 150 pg/ml (Am J Physiol., 1996, 270: R505-R517).  I’ve never seen a value as high as 1150 pg/ml and I doubt this could be obtained by stressing a rat.  My first thought on obtaining such high values would be to question the assay.  There are large differences between PTH assays from different manufacturers and some are more specific than others.

You would probably get a better idea of stress by measuring corticosterone than PTH.

Good luck with your renovations,

Mike Tordoff

________________________________
Michael G. Tordoff, Ph.D.
Monell Chemical Senses Center
Philadelphia, PA 19104, USA
________________________________
e-mail: tordoff at monell.org
phone & fax: (267)-519-4805
web: www.monell.org<http://www.monell.org/faculty/people/tordoff>
________________________________






From: rat-forum-bounces at mcw.edu [mailto:rat-forum-bounces at mcw.edu] On Behalf Of rajeshanand at lupinpharma.com
Sent: Tuesday, February 07, 2012 1:24 AM
To: rat-forum at mcw.edu
Subject: [rat-forum] PTH Levels in SD Rat
Importance: High

Dear Listers,
Could you please let me know what are the normal Parathyroid Hormone (PTH) levels in SD outbred rat ?
For the past six months , our animal facility is being renovated. Along with other mice strains,our SD rat breeding colony animals have also been exposed to construction related noise and vibrations.The scientist's have observed basal PTH levels of 1150pg/ml.I wish to know what are the reasons for elevated levels of PTH ?
Is it possible that noise/vibrations in the area surrounding SD rat animal quarter could have increased levels of PTH ?
Regards,

Rajesh K. Anand, Ph.D.,
Scientist In-charge,
Research Animal facility,
Novel Drug Discovery & Development ( NDDD),
Lupin Research Park,
46A/47A,Nande Village,
Taluka Mulshi,
Pune-411042
(Maharashtra )
India
Tel : (020)66749440
Fax ; (020)66749560



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